Effect of Temperature on Enzyme Activity

Investigating the Effect of Temperature on Enzyme Activity Almost all chemic answers that occur in sustentation organisms argon catalyzed by enzymes. Mevery factors in a jail cells environment affect the action of an enzyme. In this investigation, you will design an audition to determine the effect of temperature on an enzyme-catalyzed reaction. You will complete an entire lab write-up using the CHS LAB REPORT FORMAT. Fundamental Question How does temperature affect the rank of an enzyme-catalyzed reaction?Materials Raw colorfulForceps Petri dishresearch laboratoryeling tape and pen eye dropper pipetteIce bath 0. 1% hydrogen peroxide resolving power3 thermometers Liver pureeWarm- urine bath 25-mL graduated cylinder mea for sure or watch with second hand 5 50-mL beakersPaper towels Filter-paper disks tell apart A restrain the Catalase Reaction 1. Put on apron and condom goggles. consumption forceps to place a small piece of raw liver in an open petri dish. Use a dropper pipette to assign a drop of hydrogen peroxide solution on the liver.CAUTION total heat peroxide can be irritating to skin and eyes. If you spill any on yourself or your clothes, wash it off immediately and tell your teacher. adopt what happens. interpret in notebook. Liver contains the enzyme catalase, which breaks fine-tune hydrogen peroxide (H2O2) to water (H2O) and oxygen gas (O2). When hydrogen peroxide is broken down by catalase, bubbles of oxygen gas are released. 2. With your teachers guidance, select the right-hand(a) equipment and technology to measure catalase activitya filter-paper disk. . To measure the activity of catalase, habit a graduated cylinder to place 25 mL of hydrogen peroxide solution in a 50-mL beaker. 4. Use forceps to dip a filter-paper disk in liver puree. Place the filter-paper disk on a paper towel for 4 seconds to remove any excess liquid. 5. Use the forceps to place the filter-paper disk at the bottom of the beaker of hydrogen peroxide solution . Observe the filter-paper disk, and record the number of seconds it takes to float to the top of the liquid.RECORD in notebook. Part B Design an Experiment 1. Use your observation and knowledge to develop a TESTABLE QUESTION and about how temperature will affect the rate at which catalase breaks down hydrogen peroxide. RECORD in notebook TQ in WhenWill fix up 2. release a BACKGROUND section of the lab by doing about research about enzymes. Use part A of this lab, and the reading in section 2-4 of the textbook to help you write this.Be sure to include the biological significance (why does it matter how enzymes function why is it essential to rid the cell of H2O2 why is enzyme specificity important to the speed of the reaction), the Content of the class (what is the enzymatic reaction what are the products, reactants, and enzymes involved in the chemical reaction what do you support to happen to the speed of the reaction when temperature is manipulated what do enzymes do in che mical reactions), and the Link to this lab (how will you mental visitation this in lab what are the mv, rv, and cvs in the lab).RECORD in notebook in paragraph format. 3. Write a HYPOTHESIS to include your prediction of what will happen in the lab. RECORD in notebook HYP in IFTHENBECAUSE format. 4. Design an experiment to test your hypothesis. Your experimental plan should include PROTOCOL (materials and procedure in pictures) as well as any appropriate controls and replications (repetitions). Be sure to mention all manipulated, responding, and controlled variables in your experimental plan. Include any necessary sanctuary precautions and seriousty equipment in your plan. . As you plan your investigative procedures, refer to the Lab Tips box on p. 55 of your textbook for information on demonstrating safe practices, making wise choices in the use of materials, and selecting equipment and technology. 6. Construct a information table similar to the one below in which to record th e results of your experiment. practice you experiment ONLY after you have obtained your teachers eulogy of your plan. RECORD information in your notebook. Data Table Temperature (oC)Time (seconds)Observations Trial 1Trial2Trial3Control Temperature 7. take in a graph of the results of your experiment. Plot temperature on the x-axis and the variable by which you metrical catalase activity on the y-axis. RECORD the graph in your notebook at a lower place the psychoanalysis section. pardon this graph. 8. Make a graph of class results in the ANALYSIS section. Explain this graph. In addition to your graph, write the rest of the ANALYSIS section of the lab, using the CHS LAB REPORT FORMAT. Include the following in your notebook Explain what should have happened Identify what should have happened including the relationship amidst the time it takes the disk to float to the top and the amount of catalase activity the optimal temperature for the enzyme the shape change of enzymes whe n not in homeostasis and how that changes the effectiveness of the enzyme. Explain what echtly happened Summarize data Explain why these results occurred by incorporating background information Again, use the information from the background section (enzyme structure, enzyme function, homeostasis, temperature, pH, etc) to explain the actual results.Compare actual to expected results (describe and explain similarities and differencesuse numbers) Use simple statistics to explain data Be sure to use both extremes represented (coldest, hottest, fastest, slowest) and be sure to use averages (group and class) calculate percent change if appropriate State possible human errors and how these affected the data What did go wrong, what couldve gone wrong, explain Explain how the experiment couldve been done antithetically Did this lab protocol actually test the testable question? Were the controlled variables actually controlled? What couldve been controlled reveal? Was the experimental co ntrol actually controlled? 9. Write a CONCLUSION for the lab, again referring to the CHS format. RECORD in your notebook and be sure to include the following Re-state the hypothesis and indicate whether it was supported or rejected Did this protocol answer the fundamental question? What controlled variable(s) could be changed to better answer the fundamental question? (Describe at least two different ones)